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海洋科学 2013
Isolation, identification and lysis properties analysis of a Vibrio parahaemolyticus phage VPp1
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Abstract:
In order to develop an effective bio-controlling method against Vibrio parahaemolyticus, a virulent phage VPp1 was isolated from aquatic sewage water and identified by plaque shapes, restriction, electronic microscope and lysis properties analyses. The results revealed that VPp1 contains a linear double stranded DNA with 15kb in size and it has an icosahedral head with 44 nm in diameter and does not have a tail, suggesting that VPp1 belongs to the Tectivirus family. VPp1 forms plaques with transparent center and large halo after culture in double-layer agar plate for 12 hours and its optimum MOI value was 0.0001. A one-step growth experiment showed that the latent period and burst size were estimated at 10 min and 90.3 phage particles/infected cell, respectively. VPp1 could be widely used because of its short latent period and large burst size.