Study on influencing factors of immobilized microorganisms for remedying oil-contaminated soil
固定化微生物修复石油污染土壤影响因素研究

Aiming to remedy the oil-contaminated soil, high-efficiency oil-degrading single strain SM-3 which had been screened in the laboratory was elected as experimental strain. Use natural organic materials as carriers to obtain immobilized microorganism by adsorption method. Free and immobilized microorganisms were applied to simulate and remedy the oil-contaminated soil in the indoor pot. The orthogonal experiment of five factors (C/N/P, inoculated quantity, oil content, oxidant and surfactant) and four levels was designed to investigate the sequence of the importance of each influencing factor in different remedial periods and the remedial effect of each strain under their respective optimum conditions. The experiment showed that for different microorganisms, the importance of each factor changed in different periods. After the remediation of 21 days, the oil degradation rate of immobilized single strain SM-3 was 22.77%. During the biodegradation, inoculated quantity was the most important factor. Nutrients nitrogen and phosphorus had a relative greater effect on degradation rate compared with the influence of oxidant and surfactant.