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吉林农业大学学报 2011
The Primary Study on Construction of RNAi Expression Vector of sbe2a Gene and Maize Transformation
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Abstract:
cDNA segment of corn starch branching enzymes gene sbe2a was cloned by PCR technique and inserted into pMD18-T vector.The recombinant clone was detected by PCR and the sequence and fragments of restriction enzymes were analyzed.The results showed that the length of the clone sequence was 562 bp.The sense and anti-sense fragments of clone sequence were inserted into downstream of 35S promoter of pCAMBIA1301 to construct over-expression RNAi vector.And then it was transformed into corn inbred lines TIE 7922 b...
