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吉林农业大学学报 2011
Rapid Mini-Preparation of Plasmid DNA for Eliminating False Positive Clones
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Abstract:
A rapid method was developed to greatly simplify the procedures of plasmid DNA purification within about 15 min and greatly reduce the experiment cost compared with traditional method. Seven recombinants were constructed and identified by PCR in order to confirm reliability of the new method.The results indicated that this new method was simple and inexpensive and suitable for gram-negative bacterium.It can be used for rapidly eliminating false positive clones.
