Microplate chemiluminescence immunoassay for determination of triiodothyronine in human serum
微孔板化学发光免疫分析法检测人血清中三碘甲状腺原氨酸

A microplate chemiluminescence immunoassay(CLIA) was developed for the clinical determination of Triiodothyronine(T3) in human serum,and the luminal-hydrogen peroxide was used as chemiluminescence system catalyzed by horseradish peroxidase(HRP).Several physicochemical parameters such as the dilution ratios of coated antibody and HRP-T3 conjugate was chose,the incubation time and detection time were optimized.Methodology evaluation was done:the detection limit was 0.07 ng/mL;intra-assay and inter-assay coeff...