Honey adulteration detection using liquid chromatography/ elemental analysis-isotope ratio mass spectrometry
液相色谱/元素分析-同位素比值质谱联用法鉴定蜂蜜掺假

A new method for honey adulteration detection using liquid chromatography/elemental analysis-isotope ratio mass spectrometry (LC/EA-IRMS) was developed. Based on the individual δ13C values detected for 38 authentic honey samples, the limits for the authentic honey samples were proposed: the δ13C difference between protein and honey (Δδ13CP-H) should be higher or equal to than ~0.95‰, the δ13C difference between fructose and glucose (Δδ13CF-G) should be from ~0.64‰ to 0.53‰, and the maximum difference of δ13C values between all the components (Δδ13Cmax) should be lower than 2.09‰. Based on the above criteria, the 58 positive samples spiked with C4 or C3 plant sugar syrup were confirmed by LC/EA-IRMS method from 150 commercial honey samples, while only 7 samples spiked with C4 plant sugar syrup were confirmed by the official EA-IRMS method. The proposed method represents a significant improvement in comparing with the official EA-IRMS method.