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色谱 2010
Simultaneous determination of the five alkaloids in Rhizoma Coptidis by nonaqueous capillary electrophoresis
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Abstract:
A method for the simultaneous determination of berberine,palmatine,jatrorrhizine,magnoflorine and coptisine from Rhizoma Coptidis samples based on the nonaqueous capillary electrophoresis (NACE) mode has been developed.The effects of several important factors,such as nonaqueous solvents,running buffer system and its concentration and pH,separation voltage,temperature and detection wavelength,were investigated to acquire the optimum conditions.The optimum conditions for the separation were as follows:the sel...
