Bovine neonatal pancytopenia - Comparative proteomic characterization of two BVD vaccines and the producer cell surface proteome (MDBK)

By SDS-PAGE and mass spectrometry, we were able to detect several coagulation-related and immune modulatory proteins, as well as cellular and serum derived molecules being shared between the associated vaccine and MDBK cells. Furthermore, the number of proteins identified in the BNP related vaccine was almost as high as the number of surface proteins detected on MDBK cells and exceeded the amount of proteins identified in the non-BNP related vaccine over 3.5 fold. The great amount of shared cellular and serum derived proteins confirm that the BNP associated vaccine contained many molecules originating from MDBK cells and vaccine production.The respective vaccine was not purified enough to prevent the development of alloantibodies. To narrow down possible candidate proteins, those most likely to represent a trigger for BNP pathogenesis are presented in this study, giving a fundament for further analysis in future research.Bovine neonatal pancytopenia (BNP) is a disease transferred by colostral alloantibodies binding to peripheral blood-derived leukocytes and platelet antigens of calves [1]. Remarkably, calves develop a severe thrombocytopenia and leukocytopenia within few hours after passive transfer of colostral antibodies to blood and die within several days from bleeding disorder and bone marrow depletion [1,2]. Respective alloantibodies responsible for BNP can develop in cows previously vaccinated with a specific Bovine Viral Diarrhoea (BVD) vaccine (PregSure BVD; Pfizer, Berlin, Germany; vaccine A) [1]. Colostra of these cows transfer BNP to healthy calves, indicating a commonly expressed target antigen in responding calves [2]. Alloantibodies are also detectable in blood of respective BNP dams [1], suggesting their development to be systemically and not directly in udder. Further immunological characterization of these antibodies revealed that they were of IgG1 subclass [3]. IgG1 antibodies reflect a Th2-response in cows. So far, Major histocompatibility comple