Pharmacokinetic study on pradofloxacin in the dog comparison of serum analysis, ultrafiltration and tissue sampling after oral administration

After oral administration of enrofloxacin (5 mg/kg) and pradofloxacin (3 mg/kg and 6 mg/kg, respectively), serum collection and ultrafiltration in regular intervals over a period of 24 h were performed, followed by tissue sampling at the end of the third dosing protocol (pradofloxacin 6mg/kg). Peak concentrations of pradofloxacin (3mg/kg) were 1.55+/-0.31 mug/ml in the ISF and 1.85+/-0.23 mug/ml in serum and for pradofloxacin (6mg/kg) 2.71+/-0.81 mug/kg in the ISF and 2.77+/-0.64 mug/kg in serum; both without a statistical difference between ISF and serum. Comparison between all sampling approaches showed no consistent pattern of statistical differences.Despite some technical shortcomings the ultrafiltration approach appears to be the most sensitive sampling technique to estimate pharmacokinetic values of pradofloxacin at the infection site. Pharmacokinetics -- Pradofloxacin -- Ultrafiltration -- Dog -- Oral Administration