A study of biofilm production by gram-negative organisms isolated from diabetic foot ulcer patients

The present study was undertaken to study the difference in antibiotic resistance profile and minimum antibioticconcentration (MIC) of biofilm producing and non-biofilm producing gram-negative bacilli isolated from diabeticfoot ulcer (DFU) patients in a tertiary care hospital in North India. Among the diabetic foot patients, 73.6% weremales and 15% were females. 77.1% had T2DM whereas only 24.4% patients had T1DM. Poor glycemic controland poor HbA1c (>8) was observed in 68.7% and 70.1% patients respectively. Among the 57 patients, 97 gramnegativebacilli were isolated in which mixed bacterial infection was found in 67.8% and monomicrobial in 32.2%only. Escherichia coli was the most common (42.2%) isolate followed by Pseudomonas aeruginosa (23.7%),Klebsiella oxytoca (11.3%), Klebsiella pneumonia (9.2%), Proteus vulgaris (5.1%), Acinetobacter sp (5.1%),Proteus mirabilis (2%) and Morganella morganii (1.0%). 77.1% DFU patients had infection by biofilm producingorganisms. BFP positive status was associated with the presence of neuropathy (O.R. 7.65), osteomyelitis (O.R.3.14), duration of ulcer (O.R. 25.7), grade of ulcer (O.R. 9.12), necrotising ulcer (O.R. 14.4) and ulcer size >4cm2(O.R. 3.30) but not with patients characteristic, type of diabetes and type of diabetes, or duration of hospital stay.Poor glycemic control in 56.1% patients, amputation (24.5%), hospital stay (38.5%) and age distribution wereindependently associated with risk of biofilm producing infection in diabetic foot patients.