DINAMICS OF CYTOKINES PATTERN IN PATIENTS WITH PRESUMPTIVE AND DIAGNOSED AUTOIMMUNE DISORDERS

The purpose of the study was to determine the dynamic of cytokinic pattern in patients with suspicion or diagnosed with autoimmune illnesses in different stages, and the potential of these biological assays as possible indicators in the diagnosis and the monitoring of these illnesses. The 41 patients were selected at the District Emergency Hospital and the Municipal Hospital of Ploiesti. Bioethics requirements were followed upon for patients selected for the study. The patients’ ages varied from 21 to over 81 years old. The assay of IL -1β, IL -2, IL- 4, IL-6, IL -10, IL- 12, TNF-α was performed using the immunoenzymatic method ELISA (Enzyme-Linked ImmunoSorbent Assay). Low seric concentration of C3 and Ig A have been associated with elevated seric levels of CRP, fibrinogen, CIC, IL- 1β, IL-4 and IL-6 for the majority of patients used in the study. Very high seric levels of IL- 1β were registered in cirrhosis and hepatitis, diabetes, mushroom intoxications, CVA and hemorrhage. Patients intoxicated with mushrooms and patients with rheumatism, who also had aan underlying disese, suvh osteoporosis and obesity, presented higher values of IL- 1β, IL-2, IL-4, IL-6, and IL-10. However, the determined titres for cytokines can be used in the diagnosis of some autoimmune disorders and in the monitoring of treatments, only in correlation with clinical and paraclinical complementary data.