Research: ANTIBACTERIAL AND CYTOTOXIC ACTIVITIES OF THE RHIZOME EXTRACT OF Alpinia nigra

The investigation was conducted with crude methanolic extract of rhizome of Alpinia nigra for its antibacterial cytotoxic and activities. Antibacterial activity of the extract was evaluated against various Gram-positive and Gram-negative bacteria using disk diffusion technique. For cytotoxic activity, brine shrimp lethality bioassay was performed to estimate LC50 values. In our preliminary screening, the n-hexane, carbon tetrachloride, and dichloromethane soluble fractions of the crude methanolic extract of Alpinia nigra were subjected to antibacterial activity and brine shrimp lethality bioassay. The carbon tetrachloride soluble partitionate of the methanol extract exhibited mild to moderate antibacterial activity and strong cytotoxicity having LC50 of 0.832 μg/mL.