Novas metodologias analíticas para avalia o da eficácia fotoprotetora (in vitro) – revis o

Atualmente, s o utilizadas técnicas in vitro e in vivo para a avalia o do Fator de Prote o Solar (FPS) e do Fator de Prote o de UVA (FPA). O presente trabalho prop e a revis o das técnicas para as avalia es desses fatores in vitro preconizadas por FDA (Food and Drug Administration Agency) e COLIPA (European Cosmetic, Toiletry and Perfumery Association). O enfoque maior foi direcionado à análise espectrofotométrica de solu es diluídas e à espectrofotometria de reflectancia com esfera integrada. Palavras-chave: Espectrofotometria. Fotoprote o. UVA. UVB. Revis o. ABSTRACT New analytical methods for photoprotection effectiveness testing (in vitro) – a review Currently, both in vitro and in vivo techniques are used to measure Sun Protection Factor (SPF) and UVA Protection Factor (APF). This paper reviews the in vitro techniques to test these factors, recommended by the FDA (USA Food and Drug Administration) and COLIPA (European Cosmetic, Toiletry and Perfumery Association). The main focus is on the spectrophotometric analysis of dilute solutions and the use of reflectance spectrophotometry with an integrating sphere. Keywords: Spectrophotometry. Photoprotection. UVA. UVB. Review.