Porphyrins Regulate the Metabolic and Endocrine System: Role in Generation of Warburg Phenotype, Endogenous Digoxin Synthesis and Metabolic Syndrome X with Type 2 Diabetes Mellitus

Objectives: Actinidic archaea have been related to the pathogenesis of metabolic syndrome x. An actinide dependent shadow biosphere of archaea and viroids has been described in metabolic syndrome x with type 2 diabetes mellitus. Actinidic archaea have a mevalonate pathway and are cholesterol catabolizing. They can use cholesterol as a carbon and energy source. Archaeal cholesterol catabolism can generate porphyrins via the cholesterol ring oxidase generated pyruvate and GABA shunt pathway. Archaea can produce a secondary porphyria by inducing the enzyme heme oxygenase resulting in heme depletion and activation of the enzyme ALA synthase. Porphyrins have been related to metabolic syndrome x and type 2 diabetes mellitus. The role of archaeal porphyrins in regulation of cell functions, metabolism and endocrine function is discussed. Porphyrins play a key role in the generation of the Warburg phenotype, endogenous digoxin synthesis and metabolic syndrome x with type 2 diabetes mellitus. Methodology: The following groups were included in the study: - metabolic syndrome x with cerebrovascular thrombosis and coronary artery disease. There were 10 patients in each group and each patient had an age and sex matched healthy control selected randomly from the general population. The blood samples were drawn in the fasting state before treatment was initiated. Plasma from fasting heparinised blood was used and the experimental protocol was as follows (I) Plasma + phosphate buffered saline, (II) same as I + cholesterol substrate, (III) same as II + rutile 0.1 mg/ml, (IV) same as II + ciprofloxacine and doxycycline each in a concentration of 1 mg/ml. The following estimations were carried out: - Cytochrome F420, free RNA, free DNA, polycyclic aromatic hydrocarbon, hydrogen peroxide, pyruvate, ammonia, glutamate, succinate, glycine, delta aminolevulinic acid and digoxin. The study also involved estimating the following parameters in the patient population- hexokinase, porphyrins, pyruvate, glutamate, ammonia, succinic acid, serine, glycine, HMG CoA reductase, cytochrome C, blood ATP and heme oxygenase. Results: Plasma of control subjects showed increased levels of the above mentioned parameters with after incubation for 1 hour and addition of cholesterol substrate resulted in still further significant increase in these parameters. The plasma of patients showed similar results but the extent of increase was more. The addition of antibiotics to the control plasma caused a decrease in all the parameters while addition of rutile increased their levels. The addition of