IN VITRO ORGANOGENESIS OF TWO SANSEVIERIA CULTIVARS ON DIFFERENT CONCENTRATIONS OF BENZYLADENINE (BA)

In vitro regeneration of buds and shoots via organogenesis in two genotypes of Sansevieria trifasciata was established. Leaf segments (1cm x 1cm) of cv. Hahnii and cv. Lorentii were cultured on Murashige and Skoog (MS) basal medium containing 2.4-dichloro-phenoxyacetic acid (2.4-D) for 1 week, trans-ferred into MS medium without plant growth regulator (MS0) for 1 week, and then cultured to MS medium containing different concentrations of benzyladenine (BA:0; 0.1; 0.25; 0.5; 1 and 2 mg/l) for 3 consecutive passages with 4 week intervals. The results showed that BA proved to be an effective cytokinin to induce the formation of adventitious buds and shoots in two cultivars of Sansevieria trifasciata. The maximum of 12 propagules per explant of Lorentii and 9.3 propagules of Hahnii were obtained in medium with 2 mg/l BA after 14 weeks. Furthermore, regenerative capacity to form shoot buds and propagules was genotype dependent. The popagules number formed by cv. Lorentii was significantly higher than those formed by cv. Hahnii. The average shoot length formed by cv. Lorentii was also higher than those of cv. Hahnii. Shoots of both cultivars were successfully ex vitro rooted and acclimatized to the greenhouse with high survival rate (95.9 -100%).