Investigations regarding the anthropic impact on the Krebs cycle dehydrogenases system on certain wood-species in mining areas, Suceava county

The Krebs cycle, a second stage of cellular respiration that occurs in the mitochondrion of the leaf cell and consist in a multistep processes plays a central role in catabolism of organic fuel molecules. The mining extraction technologies for both underground and surface, the preparation of copper ore and barite applied in Tarnia, respectively to the sulphur in Calimani Mountain and the excess of these elements in natural environment may cause malfunction of ecosystem. The dehydrogenases of Krebs cycle can give information on the type and the duration of the effects of pollutants on the metabolic activity in leaves, to subsequent area pollution, therefore, the aim of the present study has been to determine these effects on this enzymatic system activity. For this reason, the isocitrate dehydrogenase, the -ketoglutate dehydrogenase, the succinate ehydrogenase and the malate dehydrogenase activity was determined using the spectrophotometric method with triphenyl-tetrazolium and the analysis of experimental results shows the differences from one sample to another sample of closely related species specificity, but also the effect of environmental factors.