HIGH PERFORMANCE LIQUID CHROMATOGRAPHY CHIRAL SEPARATION OF D,L-PHENYLALANINE AND D,L-TRYPTOPHAN WITH QUATERNARY MOBILE PHASE MIXTURE BY COPPER MIXED CHELATE COMPLEXATION

A simple and rapid HPLC enatioseparation of D,L-phenylalanine (Phe) and D,L tryptophan (DL-Trp) was developed. The resolution of enantiomers couples was based in the formation of mixed chelating complexes between copper (II) and L-histidine with quaternary mobile phase. The mobile phase was constituted of buffer ammonium acetate (pH=7), acetonitrile, isopentyl alcohol and isopropyl alcohol. An excellent separation of D, L couples was obtained with good selectivity and in no more than 15 min.