In vitro micropropagation of Alpinia zerumbet Variegate, an important medicinal plant, through rhizome bud explants

An ideal Micropropagation method of Alpinia zerumbet has been developed usingrhizome bud explants. Basal MS medium supplemented with 3% sucrose (w/v) differentconcentrations of BAP in combination with 0.5 mg / L of kinetin. Highest percentage (95%) ofexplants for shoot induction and multiple shoot (7.9 per explants) production were observedin the combination of 1.5 mg / L of BAP, 0.5 mg / L of kinetin. In this case, all the inoculatedexplants induced multiple shoots within 6-7 weeks of inoculation. Rooting was induced in amedium having half strength MS supplemented with 0.5mg / L of IBA. Most of the generatedshoots were successfully transferred to soil under field conditions.