An Alternative Efficient Technique For Thin Tooth Sectioning

Background: The importance of tooth sectioningis realized in disasters such as earthquake, airplanecrash investigation, terror, micro leakage studies, ageestimation etc. The objective of this study was to developa simple method to make thin sections (approximately100 mm) from freshly extracted teeth.Methods: One hundred and twenty human premolarsrecently extracted for orthodontic purpose were used forthis study. The teeth were stored in 0.5% chorlaraminefor 2 weeks and were not allowed to dry at any stageof the experiment. The teeth were thoroughly washedin distilled water teeth and then were sectionedbuccolingually from crown to the root portion.Results: A detailed embedding-cutting-mountingprocedure is described. The prepared thin groundsections were then examined under a Polarised lightmicroscope for the enamel and the dentine, as well asthe caries lesions can clearly be distinguished.Conclusion: This is an effective and efficient methodfor preparation of ground sections in which the hardtissue details are preserved.