HISTOPATHOLOGICAL RESPONSES OF THE LIVER TISSUES OF RANA RIDIBUNDA TO THE CHAMPIONS 50WP FUNGICIDE

Adult male and female frogs Rana ridibunda were exposed to 0.125x10-3mg Champion 50WP/g body weightadministrated by intraperitoneal injection, 1 injection at 2 days in a scheme of 3 weeks. The animals were kept at 4-6°C,respectively at 22-24°C in tap water tank. At the end of the experiment we observe large quantities of Pearls reagentmaterial in Kupffer cells, dilatation of blood vessels, peri-hepatocyte, peri-centrilobular, peri-sinusoidal and periportalfibrosis, an expansion of Disse spaces, presence of leukocyte infiltrates, vacuolated hepatocytes with small pyknoticnuclei, and necrotic areas in the parenchyma. Histological changes were more powerful at animals that were kept at 22-24oC than 4-6oC. Under normal conditions, the largest amount of copper accumulates in the cytosol. When the amountof copper is high, it accumulates in organelles: nucleus and lysosomes. Copper accumulation in the lysosomes isassociated with the initiation of detoxification process and is the beginning of its biliary excretion.