Moving towards high density clinical signature studies with a human proteome catalogue developing multiplexing mass spectrometry assay panels

The bioinformatics developments play a key central role in the area of clinical proteomics where targeted peptide expressions in health and disease are investigated in small-, medium- and large-scaled clinical studies.An outline is presented describing applications of the selected reaction monitoring (SRM) mass spectrometry assay principle. This assay form enables the simultaneous description of multiple protein biomarkers and is an area under a fast and progressive development throughout the community. The Human Proteome Organization, HUPO, recently launched the Human Proteome Project (HPP) that will map the organization of proteins on specific chromosomes, on a chromosome-by-chromosome basis utilizing the SRM technology platform. Specific examples of an SRM-multiplex quantitative assay platform dedicated to the cardiovascular disease area, screening Apo A1, Apo A4, Apo B, Apo CI, Apo CII, Apo CIII, Apo D, Apo E, Apo H, and CRP biomarkers used in daily diagnosis routines in clinical hospitals globally, are presented. We also provide data on prostate cancer studies that have identified a variety of PSA isoforms characterized by high-resolution separation interfaced to mass spectrometry.Today's health care system is in a state of major restructuring and change. We envision a considerable shift in the paradigm of how and when we meet disease within the clinic due to both growing demand from an increasing number of patients as well as the ever escalating costs for providing resources to meet these needs. This is a global problem and actual shortcomings within our societies are realized on all continents and lifestyles.For many common diseases, such as cancer, diabetes, neuro-degenerative and cardiovascular diseases there is an unmet need for diagnosing early indications of disease that could enable medical intervention and early treatment. At the same time as this is posed as one of the biggest challenges in modern health care, a novel opportunity is being created to b