Plasma Cytokines and Trace Element Level in Severe Burn Rat Model-With Special Reference to Wound Healing Potential of Ampucare

The present study was designed to determine the status of trace elements (Zn and Cu) and cytokines (IL-6, IL-1β and TNF-α) levels in severe burn rat model. Under brief anesthesia of ketamine, the shaved dorsum of the rats was exposed to 80°C (burn group) by hot wax for 5 min. The rats were fed standard pelleted diet and water ad libitum. The test room was air conditioned with temperature 23±2°C, humidity 65±5% and with artificial fluorescent light (10-14 h of light and dark), respectively. Rats were decapitated 24 h after burn injury and blood were taken for the determination of Zn, Cu and cytokines level. Present finding showed that level of Zn and Cu concentrations showed a significant trend of decrease in burned group and its levels were statistically increased (p<0.001) on 1, 7 and 14 days in ampucare treated group. Alternately, there was significant increased (p<0.01) in TNF-α, IL-6 and IL-1β in burned group on 1, 7 and 14 consecutive days as compared to control group. These levels become significantly lowered (p<0.001) on first, 7 and 14 days of treatment and come back to normal level when compared burned group. It was concluded that ampucare maintained levels of Zn and Cu as well as cytokines (IL-6, IL-1β and TNF-α) and increases its wound healing potential that would be a beneficial for the human who suffer from severe burn injury.