Gastro Protective and Antioxidant Activity of Solanum nigrum Linn. against Aspirin and Cold Restraint Stress induced Ulcerated Rats

Present study investigated gastroprotective and antioxidant activity of Solanum nigrum Linn. using aspirin and cold resistant stress induced ulcer rats. The collected plant leaves extracted with methanol by Soxhlet method for further analysis. Test animals were acclimatized in laboratory conditions and divided into six groups (Positive control (aspirin treated), negative control (normal), S. nigrum Extract (SNE) administered and aspirin, Cold Restraint Stress (CRS), S. nigrum with cold restraint stress and famotidine (standard drug) administered). The test animals were treated upto 20 days, after treatment gastric juice obtained from stomach used for biochemical and enzymatic analysis. The standard methodologies were adopted to found the parameters such as level of protein, carbohydrate, ulcer index, % of inhibition, ulcer severity, DPPH scavenging activity and reducing sugar etc. The gastric walls of all groups were used for histopathological studies. From the findings the groups that were treated to aspirin and CRS produced less gastric mucus secretion in gastric juice (101.39 and 91.67 mg g-1, respectively). The groups pretreated with S. nigrum extract exhibited a percentage inhibition of about 77.85% in aspirin and 66.67% in CRS-treated, groups, respectively. However, in groups that were pretreated with famotidine the percentage inhibition was 88.91%. Higher percentage of inhibition (88.91%) observed in groups pretreated with Famotidine. The reduction capability of DPPH radicals was determined from the decreasing absorbance values at 517 nm with increasing concentration, which is induced by anti oxidants present in the extracts. The plant extracts remarkably change all the parameters screening in test animals. Biochemical, antioxidants, histological and enzymological results also show the gastro protective and antioxidant efficiency of test plant. In nutshell the plant methanolic extracts have potential drug against gastro protective and Ulcer.