Isolation of Agrobacterium tumefaciens from soil and Optimization of Genomic & Plasmid DNA Extraction

Recent studies of Agrobacterium tumefacien species impartants in research the soil born bacterium one of the most impotant species of Agrobacterium genus can cause crown gall in plants. Parasite DNA induced to the host genome resulting in unsightly tumors and changes in plant metabolism. A. tumefaciens prompted the first successful development of a biological control agent and is now used as a tool for engineering desired genes into plants. The applied techniques such as isolation, characterization, and molecular aspects of Agrobacterium species, optimization both plasmic and genomicDNA through electrophoresis additionally, we investigated procedure can be completed within few hours accompany with fast DNA extraction materials.