Analysis of seminal quality, a tool in fertility experimental toxicology study

The methods of semen quality valuation in artificial insemination and research were suffering a constant development to estimate with more precision the males fertility. The objective of this work is making of a theoretical-practice guide that allows to carry out studies of the semen in rabbits, (New Zealand White breed), applied to the fertility toxicology thematic. Extensive results are obtained in our research in function of the female reproductive toxicity and the male in indirect form, this study allows to detect the absence or presence of reproductive toxicity directly in the male, to highlight that the results go or not more there is of the integrity of the sexual organs, anatomopathological exams, health, sexual libido, number of pregnant females, number of breedings and other variables. You should be formed 4 experimental groups from 8 to 10 rabbits males/group (32-40 total), of 7-8 months of age (adults) of 1,8-2,5 kg of corporal weight, forming a control vehicle group and three levels of the product to evaluate, being administered in repeated dose during 8 weeks (60 days for oral way, which embraces a spermatic cycle and of the following one, in the rabbit this cycle lasts 48 days), during this time it should weigh the animals weekly to determine the product doses to administer and they were observed twice a day. You can include or not a positive control group administered with reference substances, which can be administered by dose and different way to the assays substance. After 8 weeks it should extract the semen for their macroscopic and microscopic valuation with the use of an estrogenic female rabbit (1 female/6-8 male). For a bigger valuation of the toxicity product to evaluate in the male's reproductive system should carry out the anatomopathological study of organs and cavities, to extract (testicles, epididymis, prostate and seminal vesicles) for their histological study and the determination of the relationship of organ weight/corporal weight. All the analyzed variables should be compared against negative control group. This work is not based on the implementation of new techniques, but the researchers should knows that these tools exist and that they can be used to solve in an immediate way a problem given with methodologies that include a low cost, easy reproduction, quick obtaining of the results, they don't need of sophisticated teams, neither of qualified personnel in this stuff.