Transplacental micronucleus assay in rodents, a good option in experimental toxicology

The transplacental micronuclei assay has been developed aimed at evaluating the genotoxic potential in the descendants, demonstrating the capacity of an agent for causing cromosomal damages during the prenatal period. The registration of the cromosomal aberrations shows when a specific substance may be clastogenic or aneugenic in the fetus through the maternal exposure. Reason why presently work had as objective to establish a protocol for the study of transplacental micronucleus, linking this way the genotoxic and reproductive effect of a drug to evaluate for this methodology. We include the animals characteristics, the mating, administration and dosage, euthanasia method, peculiar methods of work with the sample from their obtaining (mother and fetus micronucleus), as well as the statistical analysis of the variables to analyze. Let us hope it is of the utility for all the professionals that are devoted to the experimental toxicology.