Some considerations about the development of technique for the in vivo comet assay in leukocytes of peripheral blood and liver cells

In 1988, Singh and collaborators developed the alkaline variant of the individual cells electrophoresis (comet assay), providing for the first time the data at level of individual cell. This article had as objective to provide of the researchers that they work carrying out the genotoxic evaluations some considerations about the experimental technique of the comet assay starting from leukocytes of peripheral blood and liver cells. We kept in mind for each procedure the solutions to prepare, experimental procedure and evaluation.