Incidence of metallo-betalactamase producing pseudomonas aeruginosa in Kesar SAL Medical College and Hospital, Ahmedabad

Metallo-betalactamase producing pseudomonas aeruginosa have been reported to show resistance to carbepenem drugs. Detection of metallo-betalactamases producing pseudomonas is now important to prevent their spread as dissemination of these bacteria could be fatal to the patients. The present study was undertaken over period of six months from November-2010 to May-2011 in Kesar SAL Medical College & Research institute, Ahmedabad to study the incidence of MBL producing pseudomonas aeruginosa from various clinical specimens. 100 isolates were obtained from different clinical samples of patients. These isolates were subjected to susceptibility testing to antipseudomonal drugs as per CLSI guidelines they were further screened for production of MBL by two methods i.e. EDTA impregnated imipenem double disc synergy method and Imipenem – EDTA combined disc test. Out of hundred isolates 55 imipenem resistant isolates were screened for MBL production. 38 isolates showed MBL production. MBL production was found to be 9.09%. This presents therapeutic challenge to the clinicians and also needs proper selection of antibiotics especially carbapenems.