Studies on Red-Shift Rules in Fluorescence Spectra of Human Blood Induced by LED

The characteristics of fluorescent spectrum of human blood induced by LED light have been studied in this paper. The results show that the fluorescent peak appears natural exponentially shift toward longer wavelength with the increment of blood concentration. The mechanism of energy absorption between fluorophores under different blood concentrations has been analyzed. Moreover, energy transformation between different fluorophores under the same blood concentration but different fluorescent areas has been studied. The study reveals that absorption is the main reason of red-shift.