Increasing of survival rate to Acipenser persicus by added Clinoptilolite zeolite in acute toxicity test of ammonia

The experiments were done to increase the survival rate to Acipenser persicus by added Clinoptilolite zeolite in lethal concentration of ammonia. Twelve fishes with average weight 26 ± 3 g were exposed to different concentrations (5, 15, 25, 35 mg L-1) of total ammonia salt. A group of fish was considered as control. Under stable condition, the lethal concentration of total ammonia was 35 mg L-1 during 96 hours. In the lethal concentration of total ammonia different amount of 4, 8, 12 g L-1 Clinoptilolite zeolite were used. Results indicate significant differences between treatments with each other and also control (p<0.05). With increasing zeolite in each treatment, the survival rate of fish also increased significantly (p<0.05). By addition of 12g L-1 concentration of powdered zeolite, the mortality of fish could be prevented after 96 hours. Histopathological findings showed that major lesion were hemorrhage, hyperemia, hyperplasia and epithelial cells necrosis. Also observed was degenerated tubules of kidney, expansion of Bowman’s capsule and hepatocytes necrosis by observations.