The morphologic changes in the wall of the human urinary tracts after direct electro pulse exposure: the in vitro research

The aim – study of the morphological changes in the wall of the human urinary tracts, developing after direct electro pulse exposure (DEPE). Materials and methods – The fragments of the surgical material obtained after the pelvis (n=25), ureter (n=33) and urinary bladder (n=20) open operations, were used in the research. DEPE was carried out by single (from 1 to 20) nanosecond pulses with the power of 0.8 J (joule) and 1.0 J made by the electro pulse “Urolit-105M” lithotriptor probe on the fragments’ mucous tunic placed in the Petri dishes with 0.9% NaCl solution. The fragments were fixed in the formalin and after the processing, and pouring with paraffin, the tissue slices were prepared. Results – The kidney pelvis wall, where changes like epithelium desquamation, the basal membrane disorganization, paretic capillary expansion did not go beyond sub mucous layer even after exposure by 10 pulses with the power 1.0 J each, aimed at one point, proved to be the most resistant to DEPE. In the fragments of ureter and urinary bladder the same changes were detected after exposure by 5 pulses with the power of 1.0 J each, whereas after exposure by 10 pulses with the power 1.0 J each the changes occurred which were restricted by the muscular layer. Conclusion – The DEPEon the mucous tunic of the isolated urinary tracts fragments by the means of 10 pulses with the power of 1.0 J each looks safe, causes morphologic changes, restricted by the muscular layer and does not lead to the wall perforation.