Simultaneous Determination of Ritonavir and Atazanavir in Combined Tablet Dosage Form by HPTLC

A new, simple, sensitive, precise and accurate High-performance thin-layerchromatographic method for simultaneous determination of Ritonavir andAtazanavir in their combined tablet dosage form has been developed,validated and used for determination of the compounds in commercialpharmaceutical products. Chromatographic separation was achieved onaluminium plates precoated with silica gel 60 F254 as the stationary phase andchloroform: ethyl acetate: acetone (5:2:3, v/v/v) as a mobile phase.Densitometric measurements of their spots were achieved at 244 nm overthe concentration ranges of 800-2800 ng spot-1 and 2400-8400 ng spot-1, withmean recoveries of 98.57 ± 0.35 and 99.16 ± 0.20 for ritonavir and atazanavirrespectively. Limit of detection for ritonavir and atazanavir were found to be300 ng spot-1 and 200 ng spot-1 respectively.