Synthesis and Antimicrobial Ctivity of 3-(4-(4-chlorobenzyloxy)phenyl)-1-phenyl-5-(2-chlorophenyl) Pyrazoline (3) on E. coli in Mice

The in vivo antimicrobial activity of synthesized compound Pyrazoline 3-(4-(4-chlorobenzyloxy) phenyl)-1-phenyl-5-(2-chlorophenyl) pyrazoline (3) against E. coli ATCC25922 strain was investigated. E. coli ATCC25922 strain was inhibited at concentration of 400 μg/mL. Acute Toxicity of pyrasoline, cleared that all treated mice with 400 μg/mL increase the survival time, and no significant differences of mortality and behavior of animals within a week could be seen between pyrasoline treated group and control group. Twelve days after infection, all immunological indicators in the blood were increased for infected mice, while for infected and treated was the same as day seven. Sixteen days from infection all immunological parameters for infected mice were increased, while for infected and treated group was decreased. The remaining mice infected with bacteria alone died. Survival rate was observed in the group of mice infected and treated with tested chemical compound.