IN VITRO MULTIPLICATION OF ROSA CANINA L.

The studies are based on vegetative multiplication of Rosa canina L., using meristematic explants. It were tested the axillary buds reactivity on different variants of MS medium, supplemented with growth regulators: two types of auxins (2,4 diclorphenoxiacetic acid and naphtalenacetic acid) and two cytokinins (kinetine and benzylaminopurine). The caulogenesis induction was based on the use of various combination of auxins and cytokinins (variants I – 0,02 mg/l 2,4 D – 2 mg/l K and variants II – 2 mg/l BAP-0,02 mg/l NAA). The evaluation of explants reactivity was the number of developed shoots encountered on initial explant. After two month from the initiation of culture, the shoots were isolated and cultivated separately on the MS medium, for roots induction. The whole plant reconstruction protocol lasted 10-12 weeks.