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Biopolymers and Cell 2013
Studies on interaction of oligoadenylates with proteins in vitro by MALDI-TOF mass spectrometryDOI: 10.7124/bc.000804 Keywords: oligoadenylates , insulin , α-interferon , MALDI-TOF mass spectrometry Abstract: Aim. To investigate the ability of core 2'-5'- and 3'-5'-oligoadenylates (OA) to interact with α-interferon – a key protein of the 2'- 5'-OAS/RNAase L system responsible for antiviral cell defense. Methods. MALDI-TOF mass spectrometry was used in the studies on protein-ligand interactions. Results. It was shown that 2'-5'-А3 and its epoxy-modified analog 2'-5'-А3-epo can bind to α-interferon in vitro. 3'-5'-tri- adenylate is also capable of binding to this protein. One to five ligand molecules can bind simultaneously to the molecule of α-interferon. At the same time, all studied oligonucleotides do not bind to insulin. Conclusions. It was established that core 2'-5'- and 3'-5'-triadenylates are capable of multiple interaction with α-interferon to form stable complexes. However, they do not bind to insulin which is not involved in the 2'-5'-OAS/RNAase L system.
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