Biomass production in male sterile and fertile genotypes of capsicum annum l.

In vitro study was conducted with different genotypes of Capsicum annum L. viz. cytoplasmic male sterile genotype (CMS), genetic male sterile genotypes (GMS), CMS-based maintainer genotype, GMS-based F1 hybrid and CMS-based F1 hybrid of chilli (Capsicum annum L ) for study of biomass production under two different tissue culture experiments, wherein all these genotypes were grown on half-strength MS basalmedium which was supplemented with 3% sucrose and 0.01mg/Litre 6-benzyl aminopurine (BAP) alone. Different parameters were studied for calculation of biomass production. In vivo biomass production in CMS/GMS male sterile genotypes of Capsicum annum L. is always more as compared to fertility restorer/fertile genotypes and similar results have been observed for in vitro growth of cytoplasmic male sterile genotype (CMS), genetic male sterile genotypes (GMS) and fertility restorer/fertile genotypes ofCapsicum annum L. Therefore, use of tissue culture techniques for production of biomass can be used as a method of co-relating in vivo results as biomass production character plays a significant role in the final fruit setting and fruit production in terms of yield of the plant. The presented study established that in vitro biomass production was maximum in cytoplasmic male sterile genotypes (CMS) and genetic male sterile genotypes (GMS), GMS based F1-hybrid genotypes as compared to other fertility restorer/fertilegenotypes of Capsicum annum L. GMS based F1 hybrid was found to produce more biomass just likegenetic male sterile genotype indicating the effect of biomass production character in CMS or GMS genotypes in the final fruit setting and fruit production in terms of yield of the plant.