PRODUCTION OF TANNASE BY ISOLATED ASPERGILLUS TERREUS UNDER SOLIDSTATE FERMENTATION

Tannase production under solid-state fermentation was investigated using isolated Aspergillus terreus. Among all agro -industrial waste material evaluated, wheat bran supported maximum tannase production. Solid material regulated the enzyme production and yield was improved with the supplementation of carbon and nitrogen sources to the solid medium. Maximum enzyme production was achieved with 1.5% Sucrose and 1.75% yeast extract. Glucose did not repress enzyme production but inorganic nitrogen sources showed little negative impact. The physiological fermentation factors such as pH of the medium (pH 3.5) moisture content (60%), incubation time (72 h) and inoculum level (3ml) played a vital role in tannase production. The enzyme production was found to be associated with the growth of the fungal culture