LIQUID-LIQUID EXTRACTION AND SPECTROPHOTOMETRIC DETERMINATION OF IRON USING ACETOPHENONE 2’,5’-DIHYDROXY THIOSEMICARBAZONE (ADHTS) AS A NEW ANALYTICAL REAGENT

Acetophenone 2’,5’-dihydroxy thiosemicarbazone (ADHTS) is proposed as a new sensitive and selective reagent for the Spectrophotometric determination of trace amount of Iron. The optimum extraction conditions were evaluated by studying various parameters like pH, solvent, reagent concentration, equilibration time and stability of extracted complex. The reagent reacts with Iron to form a yellow colored 1:2 chelate, at the pH 3.6.The complex is extracted in n-Butanol. The absorption spectrum shows maxima at 430 nm. Beer’s law is obeyed in the concentration range 1 to 8 ppm for Iron. The molar absorptivity is 646.06 L mol-1cm–1 and Sandell’s sensitivity is 0.0869μg cm-2 The Limit of Detection for the proposed method was found to be 0.561 ppm. The proposed method is highly sensitive, selective, simple, rapid, accurate, and has been satisfactorilyapplied for the determination of Iron in the synthetic mixtures, and real samples.