Screening of Asymptomatic Typhoid Carriers from Nail Samples from Roadside Food Handlers

In the present study, to screen the asymptomatic typhoid carriers and find out the emergency of drug resistant by antimicrobial susceptibility test. The 25 nail bits samples were collected from the food handlers (Roadside hotels) from the different age group people. Among 25 nail samples, 5 samples show a positive result. Positive results were identified by growing on selective media such as Bismuth Sulphite Agar media and it was further conformed by various biochemical tests such as Triple Sugar Iron Agar (TSI) test, Indole test, Methyl Red (MR) test and Voges-Proskeur test (VP) etc. In this 4 were women and 1 was men. These isolates were further tested with a number of conventional antibiotics viz: Amikacin, Amoxicillin, Ampicillin, Chloramphenical, Ciprofloxacin, Co-trimazole, Rifamycin, Gentamycin, Nalidixic acid and Tetracycline. Among these 4 isolates show MDRS and 1 isolate show resistance to Chloramphenicol and Gentamycin towards Amikacin, Amoxicillin, Ampicillin, Rifamycin, Nalidixic acid.