Survey of Layer Flocks Contamination to Mycoplasma gallisepticum in East Azerbaijan Province by Rapid Slide Agglutination (R.S.A) Method

The objective of this study was to evaluation of R.S.A. method efficiency in distinguishing M. gallisepticum and contamination rate of MG in Iran. In this study totally 300 serum samples from 20 farms collected and sent to laboratory. In lab, samples centrifuged at 2500 rpm for 5 min. Separated serums kept in Bain Marie at 56oC. Then 50 μ of serum are mixed with 50 : RSA-MG antigen on the slide and were assayed under light. Of 300 serum samples, 52 samples (17.3%) were positive, 10 samples (3.3%) were suspicious and 238 samples (79.3%) were negative reported. Finally, primarily can be conclude that R.S.A method is specific method for detection of M. gallisepticum and has minimum Err with maximum sensitivity. Secondarily can be conclude that contamination rate of M. gallisepticum in Iran is higher than standard levels and must be take measures in this field.