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Isolation of Agrobacterium tumefaciens Strains from Crown Gall Sample of Dicot Plants in Bangladesh

Keywords: antibiotic resistance , pathogenicity , Virulent , Agrobacterium tumefaciens

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Abstract:

Agrobacterium tumefaciens strains were identified isolated from six different dicot plants viz. Artocarpus heterophyllus, Tectona grandis, Terminalia arjuna, Anthocephalus codomba, Solanum lycopersicum and Rosa chinensis of Rajshahi University campus, Rajshahi, Bangladesh. The isolated strains were confirmed as Agrobacterium using different biochemical, antibiotic resistance and pathogenicity (tumor forming ability) tests in comparing with standard Agrobacterium tumefaciens (ATCC23308T). Accession No. of the strains was given according to their respective host plants. Statistical analysis shows that strains AtSl0105 and AtAc0114 were found more virulent than the other isolates in aspect of their tumor forming ability on potato discs.

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