Phylogenetic Diversity of the Causative Agents of Vibriosis Associated with Groupers Fish from Karimunjawa Islands, Indonesia

A molecular-based study was conducted to estimate the richness of the causative agents of vibriosis associated with groupers from Karimunjawa islands, North Java Sea, Indonesia. Moribound grouper fish were collected from the cage cultures and a total of 32 isolates were isolated from external wound and kidney of groupers. Based on the repetitive sequence-based PCR (rep-PCR) and Koch postulate test, eight isolates were chosen for further sequencings. On the basis of the sequence analysis, the data showed that the causative agents are closely related with Vibrio natriegen, V. oliviceaus, V. fortis, V. alginolitycus, V. harveyi, V. parahemolitycus, V. damsela and V. carchariae, respectively. Present study highlighted the effectiveness of rep-PCR in rapid grouping and estimating the richness of the causative agents of vibriosis associated with the groupers.