Cisplatin or carboplatin caused suppression in anti–oxidant enzyme defense system in liver, kidney and testis of male albino rats

The present study aimed to investigate the possible interference of cisplatin or carboplatin on anti-oxidant enzyme defense system in liver, kidney and testis of male rats. Rats were divided into three groups consisting of eight animals in each group. The rats in the first group were served as control and received 0.9% of normal saline only. The rats in the second group were received cisplatin (3 mg/kg body wt) only. The rats in the third group were received carboplatin (10 mg/kg body wt) respectively. Injections were given intra-peritoneally to rats on 1st, 3rd and 5th day of experimentation. On 45th day of experiment, animals were sacrificed by cervical dislocation. GST activity in the cytosol fraction of the tissues were assayed by using 1-chloro-2, 4-dinitro benzene at 340 nm. Catalaseactivities in the tissues were assayed by Chance and Machly method. The levels of lipid peroxidation in the liver, kidney and testes were measured in terms of malondialdehyde content and determined by using the thiobarbituric acid reagent. From the study it may be concluded that male rats treated with platinum compounds causes reduction in activities of antioxidant enzyme system with an increase in products of lipid peroxidation in various tissues of rats which ultimately leads to cellular peroxidation and damage in the tissues.