Acclimatization of micropropagated plants of fox grape cv. Bord (Vitis labrusca L.) in different substrates

The substrate must promote the growth of the plants, being an important component during the acclimatization of micropropagated plants. The aim of this research was to evaluate the effects of different substrates during the acclimatization of fox grape cv. Bord . The experiment was carried out at the greenhouse during February and March of 2009. Five substrates were tested: Plantmax HT, Plantmax HT: Coconut fiber (7,5:2,5 v/v), Plantmax HT: Coconut fiber (1:1 v/v), Plantmax HT: Coconut fiber (2,5:7,5 v/v) and Coconut fiber. The process of acclimatization consisted of three phases. After 20 days of in vitro culture, it was removed the PVC film (Polyvinylchloride) that covered the flasks and after two days, flasks had lids partial opening for two hours at the growth room conditions. The plants were transferred for the different substrates and placed in a greenhouse with intermittent nebulization during 16 days proceeded by 20 days of daily manual irrigation. The best substrate for acclimatization of fox grape cv. Bord was Plantmax HT, benefitting the formation of roots and aerial part of the plants. The coconut fiber must not be used alone as substrate or in larger proportions than 25% in the mixture with Plantmax HT.