Novel technique for analyzing RNA from the actively growing culture of Escherichia coli using Agilent 2100 Bioanalyzer

Molecular characterization of pathogens (bacteria, fungal, protozoa, and virus) and other microorganisms is very critical for proper diagnosis and drug development. In this study, actively growing Escherichia coli culture was used for automated analysis of variability of the culture. Normally the pathological analysis is done through microscopic visual examination, which is the traditional way of study. This is not however, a precise methodology because it has some variability depending on a variety of factors. A more specific method of analysis is needed in order to evaluate the variability and to allow for a more precise characterization of the types of pathogens that are the causal organisms of a particular disease. A novel technique coupled with fluorescence can eliminate some variability in pathogen sampling and both techniques can further enable the study of pathogens. Significant development was achieved in advancement of equipment through refinement of technology in the last decade and there has been an enormous effort to understand the molecular mechanisms of diseases and the way that drugs interact with molecular targets. At the very beginning the focus was on the research of sequencing the human genome and identifying genes associated with specific diseases, the novel technique arrived later for identifying the pathogens by using the knowledge of the science of microfluidics. The Lab on a Chip technology utilizes microfluidics, where sample preparation, fluid handling, and biochemical analysis are performed on a microchip. Data processes are analytical and fully automated eliminating the variability seen with other methods. The Lab on a Chip technology utilizes samples of various sizes of RNA, proteins, and DNA profiles to characterize the pathogen samples collected depending upon which chip is used. Fluorescent microscopy shows the detailed similarities and dissimilarities in morphology including the size and shape. These similarities and dissimilarities are needed for characterizing the pathogens and establishing the comparative accounts among them. These techniques can eliminate the variability and errors in sampling the pathogens and facilitate a quick and efficient collection of diagnostic data in pathology of various samples.