Analysis of coumarin derivatives of citrus using high resolution gas chromatography with detection systems flame ionization mass spectrometry

Citric plants, specially orange, are of great importance in Brazilian economy, mainly Southeast region. Theessential oils from Citrus are one of the most important of raw material group of several industries, mainly forfoods, pharmaceutical and toiletry. In foods industry, orange essential oil is utilized to enhance the juice flavor andit is exported, more specific to United States (main consumer). In this work was developed an analyticalmethodology for identification and quantification of coumarins in citric plants (oils, wax) using chromatographictechniques such as high resolution gas chromatography with flame ionization detector (HRGC-FID) and massspectrometry detector (HRGC-MS). The results of HRGC-MS qualitative analyses using selective ion monitoring(SIM) indicated the presence of coumarin in the samples of essential oil and wax, and in the latter it was detectedthe presence of coumarin and psoralen. Quantitative analyses by external standard methods, internal standard andaddition of standard showed good results, specially internal standard method which showed greater repeatebilitywere statistically identical (t test and ANOVA, p = 0.95).