The Effect of the 2,4-Dichlorophenoxy Acetic Acid, Benzyl Adenine and Paclobutrazol, on Vegetative Tissue-Derived Somatic Embryogenesis in Turmeric (Curcuma var. Chattip)

The nodal explants of Curcuma var. Chattip could develop callus after in vitro culturing and transplanting within 4 weeks in MS medium supplemented with 1.0 mg/l 2,4-D, although this optimal if the media was further supplemented with 5.0 mg/l BA, obtaining the highest number of new shoots in 6 weeks. MS medium supplemented with 0.01 mg/l paclobutrazol and 15% (v/v) coconut water was found suitable for regenerating the highest number of new shoots (7.25 shoots). The number of leaves per plantlet, length of leaves, and length of petrioles were significantly reduced (p≤ 0.05) when increased concentrations of paclobutrazol and especially paclobutrazol with 15 % (v/v) coconut water. However further experimentation is required to evaluate the dose-response and the interaction between coconut water and paclobutrazol. In contrast, no significant difference in leaf width in all treatments.