DERIVADOS DEL 2-FURILETILENO INDUCEN LA MUERTE CELULAR PROGRAMADA EN CéLULAS CHO DE MAMíFERO

Aim: to evaluate the morphological and physiological changes induced by the synthesized products G1 and UC245 and the apoptotic process using CHO cell culture. Material and Methods: the Ca2+ uptake, DNA fragmentation in 1,8% agarose gel electrophoresis, nuclear morphology and mitotic index were studied as evidence of programated death cell. Results: the cytosolic Ca2+ increase and DNA fragmentation observed in association with nuclear morphology changes were consistent as molecular mark of apoptosis induced by the 2-furylethylene derivatives studied. Conclusions: these two synthesized products could confirm an inductor effect on apoptotic mechanism of this cell line.