EFFECT OF GLUCOSE CONCENTRATION AND GROWTH CONDITIONS ON THE FUNGAL BIOMASS, PH OF MEDIA AND PRODUCTION OF FUMAGILLIN BY A NON-PATHOGENIC STRAIN PENICILLIUM SCABROSUM

The aim of this study was to obtain the information on the fumagillin production by a strain Penicillium scabrosum on a selected synthetic medium, and to study the effect of different glucose concentrations and the cultivation conditions on the production of fumagillin, fungal biomass, and changes of pH in media. These parameters were observed on 7th, 14th, 21st, 28th, 35th, 42nd and 49th day of cultivation. TLC (thin-layer chromatography) and HPLC analysis (high-performance liquid chromatography) were used for confirmation of fumagillin production. Based on the results from this study, MM medium with 1% glucose and 42 days cultivation at room temperature was found to be the best synthetic medium for production of fumagillin by the strain P. scabrosum used (82.54 μg.mL-1). Growth of fungal biomass reached its maximum in MM medium with 3% glucose on 14th day of cultivation at 25 °C. An increased concentration of glucose in MM medium (up to 3%) had positive impact on biomass growth, but it negatively influenced production of fumagillin. Furthermore, increasing concentration of glucose in media resulted in decreasing of pH and consequently increasing pH of media caused decreasing of fumagillin production.