Spectrophotometric methods for the determination of thiocolchicoside in bulk and pharmaceutical dosage forms

Four simple, precise and cost effective spectrophotometric methods have been developed for the estimation of Thiocolchicoside in bulk and its pharmaceutical formulations. Thiocolchicoside shows λmax at 259.0 nm in zero derivative spectrum (Method A), 252.0 nm in first derivative spectrum (Method B) and 260.0 nm in secondderivative spectrum (Method C). Method D is based on calculation of Area under Curve (AUC) for analysis of Thiocolchicoside in the wavelength range of 254.0 – 264.0 nm. The drug follows the Beer-Lambert’s law in the concentration range of 2.5 – 50.0 μg/ml for all the methods. The methods were validated by following the analytical performance parameters suggested by the International Conference on Harmonization. All validation parameterswere within the acceptable range. The developed methods were successfully applied to estimate the amount of Thiocolchicoside in bulk and pharmaceutical dosage forms.